Contrary to traditional proteomics application using LC-MS/MS, where millions of cells are simultaneously analyzed and the observed changes are presented as a cumulative response of all cells analyzed, single-cell proteomics (SCP) analysis allows for a more detailed investigation of the changes in protein compositions and functions at the single cell level. However, to confidently characterize these changes in proteins, their functions and the diversities, many individual single cells need to be analysed. Working with individual cells raises challenges not only due to the limited sample amount, but also related to sample preparation, throughput and depth of coverage. Therefore, workflows that provide the highest sensitivity, highest proteome coverage and highest throughput would be of great interest to single cell proteomics applications. Here we evaluated the depth of proteome coverage and throughput that can be achieved for low-input and single cell samples using the Orbitrap Astral Zoom mass spectrometer.